My stay at the Rockefeller Wildlife Refuge in Louisiana was idyllic and beautiful. I lucked out and had nice 70-80 degree weather, with little bugs and low humidity. The dorms at Rockefeller were quite nice with a beautiful view. The people I met were so friendly with an old school sense of southern hospitality. I spent a few days collecting alligator embryos and observing the local wildlife.
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The dorms at Rockefeller |
I saw a few alligators lurking in the water behind me.
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An alligator peeking its head up... not too far from the field lab |
By pure coincidence, a mother alligator laid some eggs in a nest very close to headquarters. I was able to observe the nest directly and recover the eggs from it.
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Collecting eggs from a nest |
Alligator eggs have a thick and brittle shell, much like a chicken's egg except larger and hardier.
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American alligator egg nest |
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I am marking the top of the egg, to ensure the top side remains up |
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Transporting the eggs |
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At the lab outside, I began dissecting the eggs |
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Working at the field lab |
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An alligator embryo is attached to the shell |
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The embryos were between 5 and 13 days |
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Alligator mississippiensis embryos |
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A baby gator |
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Juvenile alligator |
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A baby alligator eating |
Below is a pic of an alligator embryo (after being soaked in 95% Ethanol).
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Foot, Pelvic girdle and Tail of an alligator embryo |
I took one of the older 2 week embryos and placed it with A 0.2% solution of Alcian Blue (staining for cartilage). Next, I used a 0.2% Alizarin Red solution with less than 0.2% KOH. The KOH degraded the specimen a bit too much, leaving just the vertebral column. Here is a composite photo of the alligator embryo "spine." I believe the very early rudiments of vertebrae (dark blue) are forming beside the notochord (light blue).
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Alligator embryo spine. Skeletal prep Alcian Blue staining for cartilage. |
All embryos were treated ethically.
Special thanks to Dr. Ruth Elsey at the Rockefeller Wildlife Refuge, for her all her generous help and for taking photos of the egg collection.
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